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KMID : 0613820040140030467
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Journal of Life Science
2004 Volume.14 No. 3 p.467 ~ p.471
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Isolation of Antimicrobial Substance by Produced Bacillus sp. SD-10 with Antagonistic Activity Towards Mushroom Pathogens
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Lee Sang-Won
Ryu Hyun-Soon
Gal Sang-Wan
Park Ki-Hoon
Kim Cheol-Ho
Choi Young-Ju
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Abstract
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Bacillus sp. SD-10 was investigated to develope biological pesticides for control of mushroom diseases. Bacillus sp. SD-10 showed high antifungal activity when cultured at 35～40℃ for 30～40 hrs. The culture filtrate of the bacterium inhibited the growth of mycelium of T. virens which is a kind of mushroom pathogene. On the test of inhibition of spore germination of T. virens, more than 5％ of the culture filtrate in the media inhibited completely the germination of the spores. An antimicrobial substance, UPX-1 was purified from the culture filtrate of the Bacillus. From the 1H-NMR and 13C-NMR spectrum analysis, the substance was indentifed as disaccharide composed to six carbon sugars. UPX-1 has not only strong antifungal activity against T. virens but also antibacterial activity against Pseudomonas tolaassi.
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KEYWORD
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antifungal activity, antibacterial activity, mushroom pathogene
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